Title:Occurrence of blaTEM and blaROB in Haemophilus species Causing Respiratory Tract Infections
Volume: 20
Issue: 3
Author(s): Prerna Khurana and Suchitra Shenoy*
Affiliation:
- Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka 576104,India
Keywords:
Haemophilus, ROB, TEM, respiratory pathogen, sputum specimens, Haemophilus influenzae.
Abstract:
Background: Emergence of resistance to some antibiotics in Haemophilus influenzae, a
respiratory pathogen is a cause of concern. The aim is to study the antibiotic susceptibility pattern
of Haemophilus isolates from respiratory infections with reference to beta-lactam resistance.
Methods: This is a laboratory based prospective study done in the department of microbiology in
a tertiary care center after institutional ethics committee clearance. Haemophilus influenzae isolates
from respiratory tract specimens over a period of one year were subjected to antibiotic susceptibility
tests. Beta-lactamase production was detected by nitrocefin disc. hpd gene, blaTEM and
blaROB genes were detected by PCR. The data was analysed using SPSS 11.5 version.
Results: Of the 162 isolates, 89.5% were from sputum specimens. Ampicillin resistance was seen
in 5 (3.09%) isolates. The ampicillin resistant strains were positive for beta-lactamase enzyme and
blaTEM gene. BLNAR and isolates with blaROB gene were not found.
Conclusion: In case of Haemophilus influenzae respiratory tract infection empirical treatment
with amoxicillin clavulanate or third generation cephalosporin may be the drugs of choice in our
geographic area.
