Title:Anti-hyperglycemic Properties of a Purified Proteinaceous Protease Inhibitor from Macrotyloma Uniflorum Seeds
Volume: 18
Issue: 29
Author(s): Manju Mohan and Kothanam K. Elyas*
Affiliation:
- Department of Biotechnology, University of Calcutta, 673635, Kerala,India
Keywords:
Protease inhibitor, Glucose uptake, Macrotyloma uniflorum, Purification, Characterization, Degradation.
Abstract: Introduction: Fabaceae are a rich source of protease inhibitors.
Methods: A proteinaceous protease inhibitor of 25 KDa designated as Macrotyloma uniflorum protease inhibitor
(MUPI) was isolated from seeds of Macrotyloma uniflorum and purified to homogeneity by ammonium
sulphate precipitation, DEAE Sepharose column and CNBr activated Sepharose 4B Trypsin affinity chromatography.
The purity was checked by reverse phase HPLC. Long-term type 2 diabetes can lead to various biological
complications, such as hypertension and heart-related diseases. The glucose uptake studies were carried
out with the purified MUPI and it reveals its potential to be explored as a potent anti-hyperglycemic agent.
MUPI was rendered safer on HepG2 cells after MTT cytotoxicity assay.
Results: The results of glucose uptake studies suggest MUPI to be equally efficient to that of the positive control
drug Metformin against diabetes mellitus.
Conclusion: The significance of plant-based inhibitors for modulating carbohydrate breakdown and control of
glycemic index is explored to reduce the risk factors and side effects of the available drugs.
