Title:Exogenous Hydrogen Sulfide Protects SH-SY5Y Cells from OGD/RInduced Injury
Volume: 17
Issue: 8
Author(s): L. Xin, W. Junhua, L. Long, Y. Jun*X. Yang*
Affiliation:
- Department of Neurology, XiangYa Hospital, Central South University, Changsha, Hunan 410008,China
- Department of Neurology, XiangYa Hospital, Central South University, Changsha, Hunan 410008,China
Keywords:
Hydrogen sulfide, oxygen-glucose deprivation, reoxygenation, ERK3, p38MAPK, Nrf2.
Abstract: Background: Cerebral ischemia reperfusion (CIR) injury is the main
pathological mechanism of ischemia stroke, and oxidative stress (OS) plays a vital role
during the process of CIR. Recent studies have found that OS can activate MAPK
pathway, which mainly functions as a mediator of cellular stresses. It revealed that H2S
may exhibit multiple biological functions as a neuroprotector through protecting against
OS-induced neuronal injury. But how H2S can attenuate ischemia/reoxygenation injury
through MAPK signaling is not clear. The protective mechanism of exogenous hydrogen
sulfide (H2S) on oxygen glucose deprivation/reoxygenation (OGD/R) at physiological
doses in SH-SY5Y cells were investigated in this study.
Methods: DCFH- DA probe is used to measure intracellular ROS, Real-time PCR and
Western blot are used to detect the levels of p38 MAPK, ERK3 and Nrf2. CCK-8 is used
to detect cell viability.
Results: Comparing with the normal control group, the expressions of ERK3, p38MAPK
and Nrf2 mRNA in other three groups were downregulated at the beginning of 24h. After
24h, the expressions of ERK3, p38MAPK and Nrf2 were upregulated significantly
compared with the normal control group. When the cells were pretreated with 200μM
NaHS at the beginning of 24h, the expressions of ERK3, p38MAPK and Nrf2 were
decreased much more significantly than OGD/R and NaHS groups.
Conclusion: The present study demonstrated that exogenous H2S exerts a protective
effect against OGD/R-induced injury by enhancing the activation of the ERK3, p38MAPK
and Nrf2 mRNA.
