Title:Detection of blaKPC and blaGES Carbapenemase Genes in Klebsiella pneumoniae Isolated from Hospitalized Patients in Kashan, Iran
Volume: 11
Issue: 2
Author(s): Farzaneh Firoozeh, Mehdi Aghaseyed-Hosseini, Mohammad Zibaei and Ahmad Piroozmand
Affiliation:
Keywords:
Antimicrobial resistance, carbapenemases, KPC, GES, carbapenem, Klebsiella pneumonia.
Abstract: Introduction: Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria are
among the highly antimicrobial resistant gram negative bacteria and infections due to them are
an increasingly major health problem worldwide.
Methods: In this study we have detected the blaKPC and blaGES carbapenemase genes in
Klebsiella pneumoniae isolated from hospitalized patients in Kashan, Iran. In a cross-sectional
study, a total of 181 K. pneumoniae isolates were recovered from clinical specimens during
November 2013 to October 2014.
Result: Antimicrobial susceptibility profiles were determined using disk diffusion method according
to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and
CLSI guidelines. Carbapenem-resistant K. pneumoniae isolates were identified. PCR method
and sequencing were used for detection of blaKPC and blaGES carbapenemase genes. Of the 181
K. pneumoniae isolates, 35 (19.3%) were found to be resistant to imipenem and 150 (82.9%)
were identified as MDR strains. Among carbapenems, the most resistant rate 39 (21.5%) was
seen against ertapenem using disk diffusion method. Of K. pneumoniae isolates 21 (11.6%) and
42 (23.2%) carried blaKPC and blaGES genes, respectively and 19(10.5%) carried both genes
simultaneously.
Conclusion: The data of current study revealed that the frequency of resistance to carbapenems
and production of carbapenemase enzymes especially GES type was high among clinical isolates
of K pneumoniae in Kashan, Iran.
