Title:Autologous Circulating Progenitor Cells Transplanted with Hybrid Scaffold Accelerate Diabetic Wound Healing in Rabbit Model
Volume: 5
Issue: 2
Author(s): Renjith P. Nair, Harkrishnan S. Vijayakumar, Jasmin Joseph, Kalliyana V. Krishnan and Lissy Krishnan
Affiliation:
Keywords:
Autologous cell transplantation, bio mimetic scaffolds, circulating progenitors, diabetic wound regeneration.
Abstract: Background: Currently, various approaches employed for treating diabetic
wounds face several limitations. Since diabetic ischemia-related non-healing
wounds cause economic burden and social problems world-wide, newer methods
need to be developed to address the crisis.
Objective: In this study, we tested a novel strategy of applying hybrid scaffold developed
from synthetic biodegradable electro-spun poly[Lactide-glycolidecaprolactone]
and bio-mimetic fibrin based matrix combined with autologous circulating
progenitor cells on wounds in diabetic rabbits.
Methods: The wounds created in rabbit ear were grouped into three categories: (i)
untreated open wounds [control]; (ii) covered with hybrid scaffold [test1]; and (iii)
applied with autologous progenitor cell suspension and covered with hybrid scaffold [test 2]. Replicate
wounds were explanted at 3 time periods ending 28 days, gross tissue and histological sections were
compared between control and tests. Healing parameters assessed were collagen organization, angiogenesis
and epithelial coverage. Survival of transplanted cells at the wound site was tracked.
Results: All wounds healed by 28 days; but, fastest epithelial healing and least scar formations were
achieved when wounds were applied with progenitors and scaffold together. Collagen organization and
angiogenesis were the best in Test2 followed by Test1 and was minimal in Control as compared to normal
skin. Upon cell transplantation, healed skin thickness was near normal with appendage-like structures.
Conclusion: Transplanted cells could be tracked till the end of the study through fluorescence imaging.
The transplanted cells seemed important for dermal and epidermal regeneration. Even though a mixture
of cells was transplanted, all of them can be harvested easily from autologous source and could be
committed to respective lineage within few days. Therefore, it could be a potential strategy for regeneration
of wounds in human subjects.
