Title:Structure-Function Relationships of Class D Carbapenemases
Volume: 17
Issue: 9
Author(s): Jean-Denis Docquier and Stefano Mangani
Affiliation:
Keywords:
Antibiotic resistance, carbapenems, beta-lactamases, Klebsiella pneumoniae, Acinetobacter baumannii.
Abstract: Class D carbapenemases, also known as Carbapenem-Hydrolyzing class D β-Lactamases
(CHDLs) are of increasingly high clinical relevance, as they have been found in various important human
pathogens, such as Acinetobacter baumannii and Klebsiella pneumoniae and contribute to the
evolution of these pathogens towards extensively or totally-drug resistance (XDR/TDR) phenotypes.
Essentially two main groups of phylogenetically-related enzymes have been described: one including
the acquired OXA-23, OXA-24/40, OXA-51 and OXA-58 enzymes mostly in Acinetobacter baumannii,
and the other including the OXA-48-related variants, i.e. OXA-54, OXA-162, OXA-163 and OXA-
181. In this article, the biochemical and structural features of class D carbapenemases will be discussed. Furthermore, the
mechanistic hypothesis based on recently obtained crystal structures of the native forms of class D carbapenemases and
mutants thereof, in complex with relevant substrates or inhibitors, will be critically reviewed. Finally, the mechanism of
inhibition by available inhibitors, some of which are currently in clinical development, will be discussed.
