Title: Observations of the Mechanisms of Electromediated DNA Uptake - From Vesicles to Tissues
Volume: 10
Issue: 4
Author(s): Muriel Golzio, Jean-Michel Escoffre, Thomas Portet, Chloe Mauroy, Justin Teissie, David S. Dean and Marie-Pierre Rols
Affiliation:
Keywords:
Delivery, electric field, electroporation, gene transfer, membrane, microscopy
Abstract: We discuss experimental observations of DNA uptake induced by electropermeabilisation. First we describe how experiments on giant unilamellar vesicles can be used to understand the effect of electric fields on lipid membranes and the associated transfer of DNA across the plasma membrane. We then discuss how DNA interacts with electropermeabilised cells in culture and how gene expression is correlated to observations of interactions between DNA and the cell membrane. Finally we discuss how DNA uptake occurs and can be optimised for the most medically relevant case of tissues.
