Title:Histone Deacetylase 2 Stabilizes SPARC-related Modular Calcium Binding 2 to Promote Metastasis and Stemness in Gallbladder Cancer
Volume: 25
Issue: 1
Author(s): Ji Feng and Xueyong Zheng*
Affiliation:
- Department of General Surgery, Sir Run Run Shaw Hospital (SRRSH), Affiliated with the Zhejiang University
School of Medicine, Hangzhou, Zhejiang Province, 310000, China
Keywords:
Histone deacetylase 2, SPARC-related modular calcium binding 2, deacetylation, metastasis, stemness, gallbladder cáncer.
Abstract:
Background: We aimed to investigate the relationship between histone
deacetylase 2 (HDAC2) and SPARC-related modular calcium binding 2 (SMOC2) and
the role of SMOC2 in gallbladder cancer (GBC).
Methods: The expression of HDAC2 and SMOC2 in GBC and normal cells was
detected by quantitative real-time reverse transcription polymerase chain reaction
(qRT-PCR), which was also used to detect the mRNA stability of SMOC2. The
combination between HDAC2 and SMOC2 was detected by Chromatin
immunoprecipitation (ChIP) assay. After silencing and/or overexpressing HDAC2 and
SMOC2, cell viability, migration, invasion, and stemness were respectively tested by
the Cell Counting Kit-8 (CCK-8), cell scratch, transwell, and sphere-formation assay.
Results: In GBC cells, HDAC2 and SMOC2 were highly expressed. HDAC2 combined
with SMOC2 promoted mRNA stability of SMOC2. HDAC2 or SMOC2 overexpression
promoted GBC cell metastasis and stemness. SMOC2 overexpression rescued the
negative effects of silencing HDAC2 in GBC.
Conclusion: HDAC2 stabilizes SMOC2 to promote metastasis and stemness in
gallbladder cancer.
