Title:High Level of Adropin Promotes the Progression of Pancreatic Ductal
Adenocarcinoma
Volume: 24
Issue: 6
关键词:
胰腺导管腺癌，Adropin, VEGFR2，血管生成，细胞来源的异种移植物，癌旁组织。
摘要:
Background and Objectives: Preliminary experiments have revealed the abnormally
high expression level of adropin in pancreatic ductal adenocarcinoma (PDA). This study investigated
the role of adropin in the progression of PDA.
Methods: The paraffin-embedded samples of 20 patients with PDA were obtained from the hospital
biobank, and immunohistochemistry was used to evaluate adropin expression. PDA cell lines
were cultured and treated with recombinant adropin or adropin knockdown. Cell behavior was assessed,
and the expression of phospho-vascular endothelial growth factor receptor (p-VEGFR2)
and other related proteins was detected. The cell-derived xenograft (CDX) of PDA was established,
and the effects of adropin or adropin knockdown on tumor growth were observed.
Results: The PDA cancer tissues exhibited elevated adropin protein expression compared with the
paracancerous tissues, and the expression was positively correlated with carbohydrate antigen
19-9 levels in patients. Adropin significantly promoted the proliferation and migration of PDA
cells and upregulated the expression of p-VEGFR2, Ki67, cyclin D1, and matrix metalloprotein 2
(MMP2). After the knockdown of adropin expression or blockade of VEGFR2, the above effects
of adropin were significantly reversed. Adropin supplementation significantly accelerated tumor
growth in PDA CDX; upregulated the expression of p-VEGFR2, Ki67, cyclin D1, and MMP2;
and promoted angiogenesis in tumor tissue microenvironment. However, CDX inoculated with
adropin knockdown cells produced the opposite results.
Conclusion: Adropin overexpression in PDA promotes cancer cell proliferation and angiogenesis
in tumor microenvironment by continuously activating VEGFR2 signaling, thereby creating conditions
for tumor progression. Thus, targeting adropin may be an effective anti-PDA strategy.
