Title:Viaminate Inhibits Propionibacterium Acnes-induced Abnormal Proliferation
and Keratinization of HaCat Cells by Regulating the S100A8/S100A9-
MAPK Cascade
Volume: 24
Issue: 13
Author(s): Junjie Cao, Meifeng Xu, Longfei Zhu and Shengxiang Xiao*
Affiliation:
- Department of Dermatology, the Second Affiliated Hospital of Xi 'an Jiaotong University, 157 Xiwu Road, Xi 'an City
710103, Shaanxi Province, China
Keywords:
Acne, Propionibacterium acnes, viaminate, S100A8 and S100A9, proliferation, keratinization.
Abstract:
Background: Viaminate, a vitamin A acid drug developed in China, has been clinically
used in acne treatment to regulate epithelial cell differentiation and proliferation, inhibit keratinization,
reduce sebum secretion, and control immunological and anti-inflammatory actions; however,
the exact method by which it works is unknown.
Methods: In the present study, acne was induced in the ears of rats using Propionibacterium acnes
combined with sebum application.
Results: After 30 days of treatment with viaminate, the symptoms of epidermal thickening and keratin
overproduction in the ears of rats were significantly improved. Transcriptomic analysis of rat
skin tissues suggested that viaminate significantly regulated the biological pathways of cellular
keratinization. Gene differential analysis revealed that the S100A8 and S100A9 genes were significantly
downregulated after viaminate treatment. The results of qPCR and Western blotting confirmed
that viaminate inhibited the expression of S100A8 and S100A9 genes and proteins in rat
and HaCat cell acne models, while its downstream pathway MAPK (MAPK p38/JNK/ERK1/2)
protein expression levels were suppressed. Additional administration of the S100A8 and S100A9
complex protein significantly reversed the inhibitory effect of viaminate on abnormal proliferation
and keratinization levels in acne cell models.
Conclusion: In summary, viaminate can improve acne by modulating S100A8 and S100A9 to inhibit
MAPK pathway activation and inhibit keratinocyte proliferation and keratinization levels.
