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Research Article

人参皂苷Rg1通过FSP1-CoQ10- NAD(P)H途径抑制脓毒症诱导的急性肾损伤肾小管上皮细胞铁下垂

卷 31, 期 15, 2024

发表于: 10 August, 2023

页: [2119 - 2132] 页: 14

弟呕挨: 10.2174/0929867330666230607125054

价格: $65

摘要

摘要:人参皂苷Rg1可调节脓毒症引起的急性肾损伤与死亡率升高有关。在本研究中，我们探讨了它的具体机制。 方法:用铁下垂抑制蛋白1转染人肾小管上皮细胞HK-2，脂多糖诱导其铁下垂，再用人参皂苷Rg1和铁下垂抑制蛋白1抑制剂处理。采用Western blot、ELISA试剂盒和NAD/NADH试剂盒检测HK-2细胞中铁下垂抑制蛋白1、辅酶q10、辅酶q10h2和细胞内NADH水平。计算NAD+/NADH比值，免疫荧光法测定4-羟基荧光强度。CCK-8染色和碘化丙啶染色检测HK-2细胞活力和死亡情况。通过Western blot、试剂盒、流式细胞术和C11 BODIPY 581/591分子探针评估铁下垂、脂质过氧化和活性氧积累。通过盲肠结扎和穿孔建立脓毒症大鼠模型，研究人参皂苷Rg1是否在体内调节铁上吊抑制蛋白1-CoQ10-NAD(P)H通路。 结果:LPS处理降低了HK-2细胞中铁下垂抑制蛋白1、CoQ10、CoQ10H2和NADH含量，提高了NAD+/NADH比值和相对4-羟基荧光强度。FSP1过表达通过铁下垂抑制蛋白1-CoQ10- NAD(P)H途径抑制脂多糖诱导的HK-2细胞脂质过氧化。铁下垂抑制蛋白1-CoQ10-NAD(P)H途径抑制脂多糖诱导的HK-2细胞铁下垂。人参皂苷Rg1通过调节铁下垂抑制蛋白1-CoQ10- NAD(P)H通路减轻HK-2细胞铁下垂。人参皂苷Rg1在体内调节铁下垂抑制蛋白1-CoQ10-NAD(P)H通路。结论:人参皂苷Rg1通过铁下垂抑制蛋白1-CoQ10- NAD(P)H通路阻断肾小管上皮细胞铁下垂，减轻脓毒症所致急性肾损伤。

关键词: 脓毒症，急性肾损伤，人参皂苷Rg1，铁下垂FSP1-CoQ10-NAD(P)H途径，HK-2细胞，脂多糖，4-HNE。

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