Title:Topical Nanostructured Lipid Carriers of Alpha-mangostin and Resveratrol
for Synergistic Antioxidant Activity
Volume: 10
Issue: 3
Author(s): Wipada Samprasit, Chitralada Vasarach, Praneet Opanasopit, Pornsak Sriamornsak and Benchawan Chamsai*
Affiliation:
- Department of Pharmaceutical Technology, College of Pharmacy, Rangsit University, Pathum Thani, 12000, Thailand
Keywords:
Nanostructured lipid carriers, alpha-mangostin, resveratrol, synergy, antioxidant activity, topical application.
Abstract:
Background: Nanostructured lipid carriers (NLCs) are interesting lipid-based carrier
systems for enhancing the penetration of drugs through the skin after topical administration.
Objective: Dual drug-loaded NLCs of alpha-mangostin (M) and resveratrol (R) to enhance antioxidant
activity were developed for topical delivery.
Methods: The efficacy of a combination of M and R was evaluated in terms of the antioxidant
activity. M and R were loaded into the NLCs using a high shear homogenization and ultrasonication
process. The particle size, zeta potential, and physical properties of the NLCs were observed.
The M and R loading efficiency, as well as release patterns, were examined using Franz
diffusion cells. Moreover, the antioxidant efficacy and in vitro cytotoxicity in the normal human
fibroblast (NHF) of the NLCs were evaluated as well.
Results: The results found that the combination of M and R offered synergistic antioxidant activity
and was successfully loaded into the NLCs with the size of a nanometer and negative zeta potential.
The drugs were loaded in the NLCs as molecular dispersions and slowly released from the
NLCs. Interestingly, both drugs maintained their antioxidant activity after being loaded into the
NLCs and provided a higher antioxidant activity than those in the single loading of M and R, thus
demonstrating that the incorporation of M and R into the NLCs allowed an enhanced antioxidant
activity. Moreover, a cytotoxicity study showed that the NLCs were safe and had low cytotoxicity
on the NHF cells.
Conclusion: The M and R loaded NLCs were attractive systems for the synergistic antioxidant
activity for topical application.
