Title:Danshensu Enhances Cerebral Angiogenesis in Mice by Regulating the
PI3K/Akt/Mtor/VEGF Signaling Axis
Volume: 22
Issue: 4
Author(s): Hongning Jia*, Xiaoyuan Qi, Huijun Wu and Jianping Wang
Affiliation:
- Department of Neurology, Cangzhou Central Hospital, Xinhua Road, Cangzhou 061000, Hebei, China
Keywords:
Cerebral infraction, VEGF, Danshensu, angiogenesis, MCAO, thrombiolytic therapy.
Abstract:
Background: Cerebral infraction seriously affects the life quality of patients. Danshensu
has been reported to exhibit anti-inflammatory and vascular protective effects. However, the therapeutic
function of Danshensu in cerebral vascular injury is still unclear.
Methods: Middle cerebral artery occlusion (MCAO) was used to construct the cerebral infraction
model. Wound healing and tube formation assays were used to evaluate angiogenesis in vitro. Western
blot assay was used to evaluate the activation of the PI3K/Akt/mTOR signaling pathway. The laser
Doppler scanner was used to measure the regional cerebral blood flow (rCBF) in the area around the
infarction, and the adhesion removal test was used to measure the sensorimotor function. The Modified
Neurological Severity Score was performed to evaluate the cognitive functions of mice.
Results: Danshensu promoted the proliferation of bEnd.3 cells and angiogenesis in vitro. Danshensu
upregulated the expression of VEGF through PI3K/Akt/mTOR signaling pathway in bEnd.3 cells.
Danshensu improved rCBF restoration and attenuated the behavioral deficits in mice post-MCAO/R.
Conclusion: Danshensu enhances angiogenesis through the PI3K/Akt/mTOR/VEGF signaling pathway
in a mouse model of cerebral ischemic injury.
