Title:Expression of mTOR/70S6K Signaling Pathway in Melanoma Cancer
Cells and the Effects of Dacarbazine and Metformin
Volume: 18
Issue: 2
Author(s): Marjan Hajimoradi Javarsiani*, Shagayegh Haghjooy Javanmard and Javad Sajedianfard
Affiliation:
- Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Keywords:
mTOR, 70S6k, cancer, melanoma, therapy, MTT assay.
Abstract:
Background: Melanoma, also known as malignant melanoma, is a type of skin cancer
that develops from the pigment-producing cells known as melanocytes. Melanomas typically occur
in the skin but may rarely occur in the mouth, intestines or, eye. This study aims to examine the expression
of the mammalian target of rapamycin (mTOR)/70S6K signaling pathway in melanoma
cancer.
Methods: The B16F10 cell line treated with dacarbazine IC50 and different concentrations of metformin
(0.5, 2, and 8 mM) for 24 hr and mTOR and 70S6k proteins expression were examined by
western blotting. Cell viability was measured by MTT assay.
Results: Western blot analysis showed that after different concentrations of metformin and dacarbazine
treatments, the mTOR and 70S6K protein expression significantly (P<0.05) decreased.
Conclusion: Metformin-induced repression of mTOR/70S6k axis activity disrupts B16F10 growth.
Thus, we believe that combination therapy may be a suitable potential therapeutic target for melanoma
cancer.
