Title:Bioactive Extracts of Ziziphus mauritiana Induces Apoptosis in A549 Human
Lung Epithelial Carcinoma Cells through the Generation of Reactive
Oxygen Species
Volume: 18
Issue: 1
Author(s): Om Prakash, Shazia Usmani *, Amresh Gupta, Asif Jafri , Mohammad Fahad Ullah, Shadma Wahab, Md Arshad and Sudheer Kumar
Affiliation:
- Herbal Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Dasauli, Kursi Road, Lucknow, Uttar
Pradesh, India
Keywords:
Complementary therapy, Ziziphus mauritiana Lam., HPTLC fingerprinting, A549 lung cancer, reactive oxygen species, apoptosis.
Abstract:
Background: In recent years, novel metabolites isolated from botanical sources have
been attracted much attention due to their traditional and therapeutic significance. The ethnopharmacological
studies suggest that Ziziphus mauritiana is a common remedy against several kinds of
ailments.
Objective: The current study has evaluated the MeOH extract of Ziziphus mauritiana leaves (ZME)
through physicochemical, phytochemical, and chromatographic fingerprinting analysis, which displayed
an array of biometabolites of pharmacological significance, including flavonoids.
Methods: The extract was further examined for anticancer activities, which revealed promising anticancer
properties against human lung epithelial carcinoma cells (A549) and induction of apoptosis
impart by ROS. The oxidative stress was evaluated in terms of production and accumulation of
cytosolic extent of ROS, whereas anticancer perspective was determined by MTT assay, cell morphology
analysis, followed by nuclear condensation for the examination of apoptosis induction.
Results: Finding suggests that the MeOH extract of ZME markedly exhibited promising anticancer
activity against the A549 lung epithelial carcinoma cell. The ZME was found to be most active in
the MTT assay against A549 cells, while it was less toxic to normal cells. The intracellular ROS
generation was remarkably induced by ZME, which correlated with the ability of the flavonoidrich
fractions in the MeOH extract to inhibit cell growth and might induce apoptosis.
Conclusion: The present study provides useful insight concerning the promising anticancer potential
of ZME against A549 lung carcinomas. However, the clinical correlation will be required for
its authorization and the discovery of significant and least noxious novel agents against lung carcinomas.
