Title:Genetic Polymorphism of miR-218-2 (rs11134527) in Cervical Cancer: A
Case-Control Study on the Bangladeshi Women
Volume: 10
Issue: 3
Author(s): Farhana Nazneen, Md. Shalahuddin Millat, Md. Abdul Barek, Md. Abdul Aziz, Md. Sarowar Uddin, Sarah Jafrin, Tutun Das Aka and Md. Safiqul Islam*
Affiliation:
- Department of Pharmacy, Faculty of Science, Noakhali Science and Technology University, Noakhali 3814,Bangladesh
Keywords:
Cervical cancer, miR-218-2, polymorphism, risk, association, tetra-primer ARMS-PCR.
Abstract:
Background: The prevalence of Cervical Cancer (CC) is disproportionately higher in developing
countries. It is the second most frequent cancer type among Bangladeshi women and the
major cause of morbidity and mortality. However, no previous data reported the association of
miR-218-2 gene polymorphisms in Bangladeshi cervical cancer patients.
Aim: This case-control study was designed to find the link between the rs11134527 polymorphism
in miR-218-2 and CC.
Methods: A total of 488 subjects were recruited, comprising 256 cervical cancer patients and 232
healthy females. Genotyping was conducted with the tetra-primer ARMS-PCR technique to detect
the association.
Results: The results of genotype data showed that rs11134527 was in the Hardy-Weinberg equilibrium
in both CC cases and controls (P >0.05). Overall, the polymorphism was found to be significantly
associated with an increased risk of cervical cancer with AG genotype (AG vs. GG: OR =
2.26, 95% Cl = 1.40-3.66, P = 0.0008), AA genotype (AA vs. GG: OR = 3.64, 95% Cl = 2.17-6.10,
P <0.0001), dominant model (AG+AA vs. GG: OR = 2.75, 95% Cl = 1.75-4.31, P <0.0001), recessive
model (AA vs. GG+AG: OR = 2.08, 95% Cl = 1.41-3.08, P = 0.0002), and A allele (A vs. G:
OR = 1.94, 95% Cl = 1.51-2.51, P <0.0001). All of these correlations remained statistically significant
after performing Bonferroni correction (P <0.008).
Conclusion: Our study suggests that the rs11134527 polymorphism in the miR-218-2 gene contributes
to the susceptibility of CC in Bangladeshi women
