Title:miR-1-3p/CELSR3 Participates in Regulating Malignant Phenotypes of Lung Adenocarcinoma Cells
Volume: 21
Issue: 4
Author(s): Huiwen Miao, Qingxin Zeng, Shaohua Xu and Zhoumiao Chen*
Affiliation:
- Department of Thoracic Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou,China
Keywords:
Lung adenocarcinoma, miR-1-3p, CELSR3, proliferation, migration, invasion.
Abstract:
Objective: This study presents a discussion regarding the mechanism affecting the malignant
progression of LUAD and the potential therapeutic targets, so as to provide more effective
therapeutic strategies for LUAD patients.
Methods: Expression data from TCGA-LUAD were extracted to identify target miRNA, with its
downstream target mRNA predicted using bioinformatics analysis. Gene expression in transcript
level and protein level were separately examined by qRT-PCR and western blot. Cell malignant
phenotypes were assessed via MTT and Transwell assays. Luciferase reporter plasmids carrying target
gene sequences were constructed to verify the targeting association between the target miRNA
and its downstream mRNA.
Results: miR-1-3p showed decreased expression in LUAD. Over-expressing miR-1-3p suppressed
cancer cells to proliferate, migrate and invade. CELSR3, directly regulated by miR-1-3p, presented
significantly elevated expression in LUAD and could foster LUAD cells to proliferate, migrate and
invade. The rescue experiment identified that miR-1-3p-induced inhibition on LUAD cell malignant
phenotypes could be reversed by over-expressing CELSR3.
Conclusion: This study uncovered that miR-1-3p could suppress the malignant phenotypes of LUAD
cells by targeting CELSR3, which will help to provide novel therapeutic strategies for LUAD
sufferers and new references for the targeted therapy of LUAD.
