Title:Green Synthesis of Silver Nanoparticles Using Datura metel Flower Extract
Assisted by Ultrasound Method and Its Antibacterial Activity
Volume: 17
Issue: 1
Author(s): Is Fatimah*, Habibi Hidayat, Bambang Nugroho and Saddam Husein
Affiliation:
- Chemistry Department, Universitas Islam Indonesia, Yogyakarta, Indonesia
Keywords:
AgNPs, antibacterial agent, Datura metel, green synthesis, ultrasound, silver nanoparticles.
Abstract:
Background: Green synthesis method of nanoparticles has been developed for several
years. Besides providing environmental-friendly process, green synthesis of nanoparticles using
plant extract provides synergistic effect of the secondary metabolite in such antibiotic activity. The
study with an intensification process in nanoparticles formation is also gaining great attention. This
research deals with the green synthesis of silver nanoparticles using Datura metel flower extract for
the antibacterial agent. The use of ultrasound-assisted method for the synthesis was investigated.
Methods: Synthesis of silver nanoparticles (AgNPs) using Datura metel flower extract under ultrasound-
assisted method has been conducted. Evaluation of the successful synthesis was done using
UV-visible spectrophotometry, particle size analyzer, x-ray diffraction, and transmission electron
microscopy. The prepared AgNPs were tested as an antibacterial against S. aureus, K. pneumoniae,
S. pyogenes, and E. coli.
Results: The ultrasound-assisted synthesis of AgNPs produces particles ranging from 25-70 nm in
size; meanwhile, the reflux method demonstrated the size of 50-170 nm. These particles size represents
the effect on the antibacterial activity as the ultrasound-assisted synthesized Ag NPs have
higher inhibition zone towards all tested bacteria. Subsequently, these data presented the applicability
of Ag NPs synthesis using an ultrasound method as a potential candidate for biomedical applications.
Conclusion: The profile of UV-Visible spectra and particle size analyses demonstrated the applicability
of the ultrasound technique to produce a smaller size of the nanoparticles with higher antibacterial
activity.
