Title:Exercise-Induced MicroRNA Regulation in the Mice Nervous System is Maintained After Activity Cessation
Volume: 10
Issue: 2
Author(s): Andrea Carvalho, Sonia Zanon and Guilherme Lucas*
Affiliation:
- Neuroscience and Behavior Training Program, Department of Experimental Psychology, Institute of Psychology, University of São Paulo, São Paulo,Brazil
Keywords:
MicroRNA, exercise, physical training, nervous system, skeletal muscle, spinal cord, dorsal root ganglion.
Abstract:
Background: Physical exercise can improve synaptic function and protect the nervous
system against many diseases by altering gene regulation. MicroRNAs (miRs) have emerged as vital
regulators of gene expression and protein synthesis not only in the muscular system, but also in
the brain.
Objective: Here we investigated whether exercise-induced miRs expression in the nervous and muscular
systems is activity-dependent or it remains regulated even after exercise cessation.
Methods: The expression profile of miR-1, -16, and -206 was monitored by RT-PCR in the dorsal
root ganglion, in the spinal cord dorsal and ventral horn, and in the soleus muscle of mice after 5
weeks of swimming training and after swimming exercise followed by 4 weeks of sedentary conditions.
Control animals consisted of mice that swan daily for 30s during the 5-weeks training period,
returning to the non-swimming activity for additional 4 weeks.
Results: After exercise, miR-1 was upregulated in all tissues investigated. However, the upregulation
of miR-1 continued significantly high in both aspects of the spinal cord and in the soleus muscle.
The expression profiles of miR-16, and -206 were increased only in the nervous system. However,
miR-16 upregulation persisted in the DRG and in the spinal cord after exercise interruption,
whereas miR-206 continued upregulated only in the spinal cord ventral horn.
Conclusion: Exercise training can cause long-lasting changes in the expression of miRs independently
of exercise maintenance. Spatial and temporal expression of miRs is to some extent dependent
on this activity. The data raised a new conceptual hypothesis on the biogenesis of miRs, indicating
that long-lasting and systematic exercise can potentially cause irreversible miR regulation after
activity cessation.
