Title:High Expression of miR-483-5p Predicts Chemotherapy Resistance in Epithelial Ovarian Cancer
Volume: 10
Issue: 1
Author(s): Yanisa Rattanapan, Veerawat Korkiatsakul, Adcharee Kongruang, Teerapong Siriboonpiputtana, Budsaba Rerkamnuaychoke and Takol Chareonsirisuthigul*
Affiliation:
- Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400,Thailand
Keywords:
miR-483-5p, TAOK1, chemotherapy, epithelial ovarian cancer, chemotherapy resistance, RT-PCR.
Abstract:
Background: Ovarian cancer is the most deadly cancer that requires novel diagnostics
and therapeutics. MicroRNAs are viewed as essential gene regulatory elements involved in different
pathobiological mechanisms of many cancers, including ovarian cancer.
Objective: This study examined the relationship between microRNA (miRNA) expression and response
to platinum-based chemotherapy.
Methods: Genome-wide miRNA expression analysis was conducted using Epithelial Ovarian Cancer
(EOC) tissues from 25 patients with 17 malignant tumors and eight benign ovarian tumors. Candidate
miRNAs that respond to platinum-based chemotherapy were selected for validation by quantitative
RT-PCR.
Results: Among 2,578 mature human miRNAs, high expression of miR-483-5p correlated with
poor responses to platinum-based chemotherapy in EOC patients. Furthermore, high levels of
miR-483-5p in the resistant group suppressed expression of the apoptotic regulator TAOK-1.
Conclusion: A possible marker for the prediction of chemotherapy response and resistance in patients
may be miR-483-5p. Choosing the right treatment for each patient with EOC can avoid the
risk of developing chemotherapy resistance.
