Title:Ethanol Extract of Achillea fragrantissima Enhances Angiogenesis through Stimulation of VEGF Production
Volume: 21
Issue: 11
Author(s): Hana M. Hammad*, Amer Imraish, Maysa Al-Hussaini, Malek Zihlif, Amani A. Harb, Tuqa M. Abu Thiab, Zainab Lafi, Zeyad D. Nassar and Fatma U. Afifi
Affiliation:
- Department of Biological Sciences, School of Science, The University of Jordan, Amman,Jordan
Keywords:
Achillea fragrantissima L., asteraceae, angiogenesis, aortic ring assay, VEGF, wound healing.
Abstract:
Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb
in the rural communities of Jordan.
Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis
in both, ex vivo using a rat aortic ring assay and in vivo using a rat excision wound model.
Results: In concentrations of 50 and 100 μg/ml, the ethanol extract showed angiogenic stimulatory
effect and significantly increased length of capillary protrusions around aorta rings of about 60% in
comparison to those of untreated aorta rings. In MCF-7 cells, the ethanol extract of A. fragrantissima
stimulated the production of VEGF in a dose-dependent manner. 1% and 5% of ethanol extract
of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds
for six days and found to be effective in wound healing and maturation of the scar. Both preparations
resulted in better wound healing when compared to the untreated control group and vaseline-
treated group. This effect was comparable to that induced by MEBO, the positive control.
Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act
through the VEGF signaling pathway.
