Title:Differential Expression of miR-20a and miR-145 in Colorectal Tumors as Potential Location-specific miRNAs
Volume: 10
Issue: 1
Author(s): Sara Eslamizadeh, Ali-Akbar Zare, Atefeh Talebi, Seidamir Pasha Tabaeian, Zahra Shokati Eshkiki, Hafez Heydari-Zarnagh and Abolfazl Akbari*
Affiliation:
- Colorectal Research Center, Iran University of Medical Sciences, Tehran,Iran
Keywords:
Biomarker, colon, location, microRNA, rectum, Rectal Cancer (RC).
Abstract:
Background: MicroRNAs (miRNAs), as tissue specific regulators of gene transcription,
may be served as biomarkers for Colorectal Cancer (CRC).
Objective: This study aimed to investigate the potential role of the cancer-related hsa-miRNAs as
biomarkers in Colon Cancer (CC) and Rectal Cancer (RC).
Methods: A total of 148 CRC samples (74 rectum and 74 colon) and 74 adjacent normal tissues
were collected to examine the differential expression of selected ten hsa-miRNAs using quantitative
Reverse Transcriptase PCR (qRT-PCR).
Results: The significantly elevated levels of miR-21, miR-133b, miR-18a, miR-20a, and
miR-135b, and decreased levels of miR-34a, miR-200c, miR-145, and let-7g were detected in colorectal
tumors compared to the healthy tissues (P<0.05). Hsa-miR-20a was significantly overexpressed
in rectum compared to colon (p =0.028) from a cut-off value of 3.15 with a sensitivity of
66% and a specificity of 60% and an AUC value of 0.962. Also, hsa-miR-145 was significantly
overexpressed in colon compared to the rectum (p =0.02) from a cut-off value of 3.9 with a sensitivity
of 55% and a specificity of 61% and an AUC value of 0.91.
Conclusion: In conclusion, hsa-miR-20a and hsa-miR-145, as potential tissue-specific biomarkers
for distinguishing RC and CC, improve realizing the molecular differences between these local tumors.
