Title:A Comparative Analysis of Different Enzyme Immobilization Nanomaterials: Progress, Constraints and Recent Trends
Volume: 28
Issue: 20
关键词:
酶固定化方法、纳米材料、酶稳定性、生物传感器、工业应用、基质。
摘要: Immobilization techniques have been popularly used to preserve the operational
stability of the enzymes for industrial applications. The three main components of an
immobilized enzyme system are the enzyme, the matrix/support, and the technique of immobilization.
So far, different supports have been developed to improve the efficiency of
the immobilized enzymes. But in the recent decade, nanotechnology has been of considerable
research interest in the field of immobilized enzyme carriers. The materials at the
nano-scale, due to their unique physicochemical properties, including; specific surface
area, mass transfer limitation, and effective enzyme loading, are considered interesting
matrices for enzyme immobilization. This review describes techniques employed to immobilize
enzymes, and provides an integrated focus on the most common nanoparticles
for enzyme conjugation. Additionally, the pros and cons of nanoparticles as immobilization
matrices are also discussed. Depending on the type of enzyme and its application, in
this review, the researchers are directed to select an appropriate method and support for
enzyme immobilization in terms of enzyme stability and functionality.
