Title:A Refined Framework for Precision and Translational Proteomics in Clinical Research
Volume: 18
Issue: 4
Author(s): Min Wang, Daozhen Chen, Yankai Xia, Tao Zhou* Shi-Wen Jiang*
Affiliation:
- Research Institute for Reproductive Medicine and Genetic Diseases, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002,China
- Center for Reproductive Medicine, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002,China
Keywords:
Biomarkers, functional proteomics, precision proteomics, proteomic methods, quantitation, reproducibility, translational
proteomics.
Abstract: Most clinical related proteomic studies were aimed to screen potential biomarkers for
clinical usage. However, it is known that only a few candidates can be validated in later stages. Besides
clinical biomarkers, it is also necessary to identify key proteins to better understand the molecular
mechanism of human diseases. Here, we updated the connotations of precision and translational
proteomics and proposed a refined research framework for clinical proteomics. We abstracted
the framework as a three-tier system: to determine research objectives and approaches for achieving
a certain purpose, to choose the appropriate methods for optimal results, and to apply a standardized
experimental procedure for reliable and reproducible results. Started from the initial discovery
stage, we divided the following proteomics-driven translational studies into two directions:
biomarker screening and mechanism investigation. We also discussed possible causes that may
lead to high rates of experimental variation and low rates of follow-up validation. Moreover, we
pointed out that the main bottlenecks for the identification of effective targets are data interpretation
and statistical inference. Finally, we emphasized that the transparency of experimental design
and results can help to ensure data quality. Conclusively, the refined framework provides constructive
recommendations and comprehensive guides for designing and performing clinical related proteomic
studies with the aim of obtaining reliable and reproducible results. It can also help to further
promote the standardization and integration of proteomic studies.