In Silico and In Vitro Studies of the Inhibitory Effect of Antihistamine Drug Cyproheptadine Hydrochloride on Human Salivary Alpha Amylase

Benguechoua       Madjda; Benarous       Khedidja; Nia       Samira; Yousfi       Mohamed

doi:10.2174/1871523019666201023111825

Abstract

Background: For the first time, the inhibitory effects on the human salivary alpha-amylase activity of the anti-inflammatory drugs indomethacin, diclofenac sodium, ketoprofen, diclofenac potassium, diclofenac, triamcinolone acetonide, and the antihistamine drugs levocetirizine dihydrochloride, desloratadine, cycloheptadine hydrochloride, have been investigated to confirm the other properties of these drugs.

Objective: This study aimed to determine the effect of nine known drugs on human salivary α-amylase in vitro and the nature of interactions with structure-activity relationship using molecular docking experiments.

Methods: The inhibition of human salivary alpha amylase by the six anti-inflammatory and three antihistamine drugs has been carried out using the new method that has been proved in our previous work. Molecular docking has been achieved for the first time for these drugs using the Auto- Dock Vina program.

Results: Cyproheptadine hydrochloride presented the highest inhibitory activity against α-amylase with IC₅₀=0.7 mg/ml, while the other drugs showed weak activities (IC₅₀ > 2 mg/ml).

Conclusion: We conclude that Cyproheptadine hydrochloride, which was studied by docking experiments, exhibited the best inhibitory activity on salivary α-amylase in vitro & in silico.

Keywords: Inhibition activity, human salivary α-amylase, anti-inflammatory drugs, antihistamine drugs, cyproheptadine hydrochloride, molecular docking.

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DOI https://dx.doi.org/10.2174/1871523019666201023111825	Print ISSN 1871-5230
Publisher Name Bentham Science Publisher	Online ISSN 1875-614X

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry

In Silico and In Vitro Studies of the Inhibitory Effect of Antihistamine Drug Cyproheptadine Hydrochloride on Human Salivary Alpha Amylase

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